FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l08n01_nutrinetat_vegc56_mr-0_r2o_hn.35100000089cd6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l08n01_nutrinetat_vegc56_mr-0_r2o_hn.35100000089cd6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25211883
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG3937371.5617119911273585TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG447150.041.70596315
GCACACG446700.041.70265211
TCGTATG447850.041.13289345
CGTTTCG450050.041.0624233
AGTCACG450750.041.05105628
CACACGT458800.040.62734212
AGCACAC460400.040.53012510
ACACGTC460350.040.51986713
GTCACGT460550.040.1091429
CTCGTAT467150.039.49183744
GTTTCGG469950.039.2852634
GAGCACA477100.039.2623259
CACGTTT473400.039.19587731
TCGGAAG479100.039.1088943
CACGTCT477800.039.030614
TTCGGAA476450.038.69737236
ACGTTTC479200.038.68268232
CGTCTGA482750.038.66301316
TTTCGGA479000.038.4960635
GATCGGA489450.038.428361
Produced by FastQC (version 0.11.2)