FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l07n01_nutrinetat_vegc54_mh-1_r2o_hn.3510000008a08f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l07n01_nutrinetat_vegc54_mh-1_r2o_hn.3510000008a08f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13137863
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC1067320.8124000075202489TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG139150.040.38036715
GTATGCC127950.040.26877645
GCACACG140700.039.98348211
CGTATGC130650.039.32309744
CACACGT145450.038.77052312
ACACGTC145600.038.56064213
TATCTCG133750.038.32759539
AGCACAC146950.038.2523110
TCGTATG135150.038.06371343
CACGTCT150050.037.4320514
CTCGTAT138700.036.97595642
GAGCACA152300.036.9676639
TCGGAAG153100.036.6851273
CAGTCAC155250.035.11293827
GATCGGA161150.034.9557271
CGTCTGA162100.034.73272716
TCTCGTA148300.034.6430441
ATCGGAA162850.034.56252
AGAGCAC163400.034.4976928
CATTTTA148900.034.3674834
Produced by FastQC (version 0.11.2)