FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l07n01_nutrinetat_vegc52_kn-0_r2o_hn.3510000008a059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l07n01_nutrinetat_vegc52_kn-0_r2o_hn.3510000008a059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19178817
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC1386440.722901730591621TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC161500.039.9755445
ACGTCTG169950.039.29589515
GCACACG170650.039.24015411
CGTATGC165100.039.0605944
ACACGTC177300.037.75568813
CACACGT180100.037.26861612
TCGTATG176250.036.6533343
AGCACAC184100.036.34890410
CACGTCT185650.035.9969614
TCGGAAG192550.035.0640263
CTCGTAT184700.035.0251642
GAGCACA192350.035.0003749
CGTCTGA193100.034.689716
GATCGGA199950.033.6006281
ATCGGAA202950.033.2138822
CAGTCAC199150.032.8871727
AGAGCAC205450.032.7358178
GGCATCT196950.032.73243737
ATGGCAT198550.032.5479735
CATGGCA198950.032.4486134
Produced by FastQC (version 0.11.2)