FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l07n01_nutrinetat_vegc50_ge-0_r2o_hn.35100000089fc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l07n01_nutrinetat_vegc50_ge-0_r2o_hn.35100000089fc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18632088
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG1414290.7590614642867723TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC163350.040.0712933
ACGTCTG172300.039.86971715
GCACACG173900.039.5546311
ACGTGGC166550.039.39919732
GTCACGT168450.038.98370729
CACGTGG169400.038.8039931
GCCTTAT170400.038.69061737
TCACGTG171250.038.30689630
AGTCACG174450.038.18668428
ACACGTC180950.038.08812313
TCGTATG174000.037.78962345
CACACGT182600.037.7316412
TATCTCG175650.037.6622541
GGCCTTA177550.037.1705436
AGCACAC185750.037.14021310
GTGGCCT176800.037.0864934
CTCGTAT178450.036.99567844
CACGTCT187800.036.67490814
GAGCACA196100.035.3290989
TCGGAAG196800.035.1878363
Produced by FastQC (version 0.11.2)