FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l07n01_nutrinetat_vegc48_edi-0_r2o_hn.35100000089f40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l07n01_nutrinetat_vegc48_edi-0_r2o_hn.35100000089f40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23313101
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCC12462215.345582297267104TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1319550.044.09497545
GCACACG1387000.043.84308211
ACGTCTG1388150.043.82782415
CAGGCGA1317800.043.7403734
CACACGT1392650.043.70720312
CCAGGCG1327500.043.64950633
AGCACAC1396500.043.5657710
ACACGTC1398000.043.5174113
CGTATGC1338700.043.45066544
AGGCGAT1330500.043.26875735
GGCGATC1329250.043.23837336
CACGTCT1407000.043.23745314
TCGTATG1346800.043.2060443
ACCAGGC1347700.043.12534732
GATCGGA1417600.043.1228561
TCGGAAG1416600.043.084523
CGTCTGA1412600.043.07082416
GAGCACA1413900.043.0646259
CTCGTAT1353150.042.98499742
GCGATCT1340750.042.87925337
Produced by FastQC (version 0.11.2)