FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l07n01_nutrinetat_vegc46_ct-1_r2o_hn.35100000089eba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l07n01_nutrinetat_vegc46_ct-1_r2o_hn.35100000089eba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18911084
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCC7350673.886963856751945TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG817900.043.6972211
GTATGCC786700.043.65875645
ACGTCTG819600.043.5242515
CACACGT824600.043.35308512
ACACGTC826300.043.2611713
AGCACAC829500.043.10783810
CGTATGC799800.042.95649344
CACGTCT831850.042.9238614
GAGCACA839050.042.6761679
TCGGAAG841600.042.654073
TCGTATG806400.042.5993443
CGTCTGA838300.042.5345716
CTCGTAT811300.042.3309642
GATCGGA851400.042.215971
ATCGGAA851200.042.1824342
CAGTCAC824100.042.08198527
AGAGCAC851200.042.056448
CACTCAA822800.041.84319734
CCACTCA827600.041.84240733
TCCAGTC841250.041.83119225