FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l07n01_nutrinetat_vegc44_col-0_r2o_hn.35100000089e41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l07n01_nutrinetat_vegc44_col-0_r2o_hn.35100000089e41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16927084
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC3773072.229013573749619TruSeq Adapter, Index 10 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG197250.116529226179772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC406300.043.1718545
ACGTCTG420000.042.4856215
CGTATGC413400.042.38039844
GCACACG422650.042.19794511
ACACGTC428000.041.71251713
TCGTATG420600.041.6602643
TATCTCG421450.041.62426839
AGCACAC429250.041.6067710
CACACGT429600.041.5676412
CTCGTAT423150.041.34541742
TAGCTTA426000.041.18497534
TCGGAAG435850.041.120273
CACGTCT434450.041.1087714
AGTCACT429650.041.00111428
CGTCTGA435900.040.89462316
GAGCACA438550.040.7654769
ACTAGCT430600.040.7385332
GTCACTA432100.040.6938229
CACTAGC433050.040.5143231
ATCGGAA444650.040.3153422
Produced by FastQC (version 0.11.2)