FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l07n01_nutrinetat_vegc42_bur-0_r2o_hn.35100000089dc8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l07n01_nutrinetat_vegc42_bur-0_r2o_hn.35100000089dc8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17014369
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG30200.018.3969115
GCACACG31350.017.79381411
CGAGTGG28850.017.23063933
ACGAGTG32050.015.72191732
AGTCACG34300.015.15123628
ACACGTC36850.015.138020513
CACGAGT33900.014.99700331
TATCTCG33750.014.99559241
CACACGT38700.014.53061312
TCGTATG36650.013.99826145
AGCACAC42700.013.59085310
CACGTCT41350.013.49059414
GTCACGA39850.013.09692729
GAGCACA46150.012.6723329
GCCGGTT25350.012.4390161
GGGCGCT4550.012.3755531
CGTCTGA46600.012.16380616
CTCGTAT43200.012.07978344
GCGGGCA8250.011.4665121
GCCGGCT14750.011.452631
Produced by FastQC (version 0.11.2)