FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l07n01_nutrinetat_vegc40_bi-1_r2o_hn.35100000089d35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l07n01_nutrinetat_vegc40_bi-1_r2o_hn.35100000089d35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25111232
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG16608816.6140960347943105TruSeq Adapter, Index 22 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279690.11138043724816049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1815450.043.88887411
GTACGTA1756600.043.85721235
CGTACGT1758150.043.84925534
TCGTATG1762250.043.8394945
CCGTACG1761300.043.811733
ACGTCTG1818350.043.80403515
ACACGTC1825650.043.66214413
AGCACAC1825550.043.6608410
CACACGT1826500.043.65783312
TCGGAAG1839700.043.541563
TACGTAA1767000.043.53416436
ACCGTAC1775950.043.4694732
GAGCACA1837200.043.4231579
ACGTAAT1776650.043.4141837
GATCGGA1851700.043.3641361
CACGTCT1838750.043.34251414
CGTCTGA1841200.043.26529316
ATCGGAA1855050.043.2536622
AGAGCAC1849600.043.1429868
CTCGTAT1797800.042.9772644
Produced by FastQC (version 0.11.2)