FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l07n01_nutrinetat_vegc38_akita_r2o_hn.35100000089cbc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l07n01_nutrinetat_vegc38_akita_r2o_hn.35100000089cbc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26197245
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG1964940.7500559696258138TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG239350.039.23521411
ACGTCTG241150.039.05428315
CGTTTCG236800.038.91681333
GTCACGT239850.038.4280229
AGTCACG244050.037.99954228
TCGTATG244600.037.645545
CACACGT250950.037.43055712
AGCACAC252450.037.32398210
ACACGTC252100.037.29549813
CACGTCT261450.035.98754514
CTCGTAT257650.035.8024444
GAGCACA268650.035.3579649
GTTTCGG261200.035.30723634
CACGTTT265750.034.8419431
TCGGAAG274600.034.4545673
CGTCTGA274250.034.29150416
CAGTCAC275650.033.92194727
TTTCGGA273500.033.75227435
TTCGGAA273550.033.6392136
ACGTTTC277100.033.60837632
Produced by FastQC (version 0.11.2)