FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l06n01_nutrinetat_vegc71_tsu-0_r2y_hn.3510000008a072.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l06n01_nutrinetat_vegc71_tsu-0_r2y_hn.3510000008a072.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19095124
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC1946461.019349232819855TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC219500.041.26030745
ACGTCTG231200.040.5775115
GCACACG233500.040.2934111
CACACGT236800.039.655912
CGTATGC228900.039.53095244
TATCTCG230250.039.33824539
CTCGTAT231350.039.14148342
AGCACAC240700.039.06943510
ACACGTC241200.038.95114513
TCGTATG233700.038.7190243
CACGTCT247300.037.90850414
TCGGAAG253850.037.1383673
TCTCGTA244450.037.0715141
GATCGGA255050.036.9490661
GAGCACA255600.036.923919
CAGTCAC254200.036.20692427
ATCGGAA262200.035.9921042
CGTCTGA262200.035.75428416
CATTTTA259000.035.21471434
AGAGCAC269100.035.021398
Produced by FastQC (version 0.11.2)