FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l06n01_nutrinetat_vegc69_stw-0_r2y_hn.35100000089fe0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l06n01_nutrinetat_vegc69_stw-0_r2y_hn.35100000089fe0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11871074
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC701270.5907384622486559TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC84250.039.07615745
ACGTCTG88000.038.7745615
GCACACG91150.037.43457411
CGTATGC89400.036.85904344
CACACGT93250.036.5915412
CTCGTAT91400.036.1755542
AGCACAC95700.035.7487810
ACACGTC96250.035.54450213
TCGTATG93250.035.3854943
CACGTCT97500.034.90424714
GAGCACA100500.034.1980449
TCGGAAG102350.033.406153
TCTCGTA99350.033.34869441
CGTCTGA104500.032.6953116
CAGTCAC104000.032.23330327
ATGGCAT102800.032.0763435
GATCGGA107750.031.69191
CTGAACT109900.031.17264219
GGCATCT106350.031.00562137
CATGGCA108100.030.91982834
Produced by FastQC (version 0.11.2)