FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l06n01_nutrinetat_vegc67_st-0_r2y_hn.35100000089fad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l06n01_nutrinetat_vegc67_st-0_r2y_hn.35100000089fad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25851739
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG1724320.6670034847558998TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC204150.040.4012833
ACGTCTG221800.039.07248315
ACGTGGC212200.038.97019632
GCACACG225350.038.5767411
GTCACGT215600.038.39848729
CACGTGG217850.038.09417731
GCCTTAT218650.038.03063637
AGCACAC234100.037.20210310
TATCTCG224200.037.1995541
AGTCACG224850.037.16108728
TCACGTG222900.037.1604730
CACACGT233550.037.12599612
ACACGTC235950.036.7197613
CTCGTAT226650.036.6287344
TCGTATG226850.036.56239745
GTGGCCT226300.036.4766634
CACGTCT238150.036.38055414
GGCCTTA234200.035.60157836
TCGGAAG252000.034.574293
TCTCGTA243350.034.29068443
Produced by FastQC (version 0.11.2)