FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l06n01_nutrinetat_vegc65_shahdara_r2y_hn.35100000089f19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l06n01_nutrinetat_vegc65_shahdara_r2y_hn.35100000089f19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23833156
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCC3638371.5266001699481178TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG381440.16004594607613024No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA241090.10115739602426133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC399500.042.4947545
CCAGGCG401050.042.2427833
CAGGCGA400500.042.18285434
ACGTCTG409500.042.0302315
GCACACG414100.041.73715211
AGGCGAT406450.041.42145535
CACACGT418400.041.2974612
CGTATGC412400.041.1619944
ACACGTC421700.040.88361413
ACCAGGC415550.040.8280532
AGCACAC423900.040.78816610
TCGTATG416650.040.7691243
GGCGATC413250.040.66911736
CTCGTAT419000.040.52435742
CACGTCT429850.040.0927614
GAGCACA435150.039.7491729
CGTCTGA434350.039.64631316
TCGGAAG438000.039.331283
GCGATCT431200.039.05438237
CAGTCAC441950.038.68385327