FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l06n01_nutrinetat_vegc63_sakata_r2y_hn.35100000089e77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l06n01_nutrinetat_vegc63_sakata_r2y_hn.35100000089e77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21322639
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCC267370.12539254639165442TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC44300.025.81642745
ACGTCTG49250.024.02252815
CGTATGC50600.022.3592244
GCACACG54450.021.9349111
ACACGTC57900.020.78329513
CACACGT59000.020.1289512
TCGTATG58800.019.43236243
AGCACAC63250.019.38095710
CTCGTAT61300.018.71323442
CACGTCT68200.017.54552814
TCGGAAG68750.017.3654273
GAGCACA72150.016.9590599
CGTCTGA77350.015.67355916
GATCGGA77150.015.4171721
CAGTCAC77000.015.31004727
GCGGGCT10300.015.30973151
GGGCGCT3850.015.2131591
TCTCGTA81850.014.12484341
GCGGGCA10250.014.0657481
ATCGGAA84500.013.9161312
Produced by FastQC (version 0.11.2)