FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l06n01_nutrinetat_vegc61_oy-0_r2y_hn.35100000089e0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l06n01_nutrinetat_vegc61_oy-0_r2y_hn.35100000089e0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528046
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC30740.5821462524098279TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC4050.040.17268845
CGTATGC4250.038.10457244
TATCTCG4350.037.22860739
TCGTATG4500.036.9873143
ACACGTC4450.036.89745313
ACGTCTG4450.036.3920115
GATCGGA4700.035.9429931
TCGGAAG4600.035.7416573
CTCGTAT4600.035.69427542
AGTCACT4550.035.60229528
AGCACAC4550.035.09784710
CACACGT4600.034.71634712
CAGTCAC4700.034.4693227
ACTAGCT4700.034.46278832
TCACTAG4700.034.46278830
TAGCTTA4700.034.4562634
CGGAAGA4750.034.1388244
CACGTCT4750.034.09356314
ATCGGAA4850.033.9025232
TCTCGTA4800.033.73842241
Produced by FastQC (version 0.11.2)