FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l06n01_nutrinetat_vegc59_n13_r2y_hn.35100000089d85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l06n01_nutrinetat_vegc59_n13_r2y_hn.35100000089d85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26004121
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGAT33200.014.5193091
GCGGGCA13750.013.597081
GGGCGCT4750.013.278061
GCCCGTT15150.012.3405841
GCGGGCT12850.012.0952881
GTCCGCT9700.012.0754091
CGGGCGC8950.012.06317715
GCGGGCC6000.012.0134841
ACGAGTG38950.011.83981832
ACGTCTG40900.011.76884815
GCACACG41800.011.73069511
TCGGGGG21150.011.7150742
CGCGTAT15050.011.67423251
CACACGT45550.011.15998412
GGCGAAT40100.011.122211
GCCGGTT49950.011.0484361
GCGGGTT60350.010.9733771
GCCGGCT24900.010.7650951
GCGGGAC12850.010.6929361
GGGCGCC2952.1049527E-710.6899641
Produced by FastQC (version 0.11.2)