FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l06n01_nutrinetat_vegc57_mt-0_r2y_hn.35100000089d0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l06n01_nutrinetat_vegc57_mt-0_r2y_hn.35100000089d0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31967302
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA27250.020.30548535
CGTACGT32450.017.05160134
CCGTACG37300.015.49776533
GCCGGAT41900.015.16085151
ACGTCTG46450.013.3165515
GGGCGCT6600.013.3109551
GGGCGCC2959.458745E-1112.9812171
TACGTAA47350.011.92335636
GCACACG53600.011.70803911
GCGGGTT62900.011.2810211
TCGGGGG25400.011.0856232
GCGGGCA15950.011.0159621
ACCGTAC53150.010.96188932
GCGGGCT17250.010.708121
GGCGAAT50350.010.647951
CGGGCCG4101.891749E-1010.4389851
GTCGGTT49100.010.3226111
GCGGGAT40600.010.1534491
GCCCGTT22650.010.1442631
GCGGGCC6300.010.0116581
Produced by FastQC (version 0.11.2)