FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l06n01_nutrinetat_vegc55_mr-0_r2y_hn.35100000089cc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l06n01_nutrinetat_vegc55_mr-0_r2y_hn.35100000089cc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55668908
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG4566450.8202873316645622TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG540850.039.63258433
ACGTCTG552050.039.26447315
GCACACG555100.039.09330411
CACACGT564900.038.43899512
GTCACGT558550.038.354529
AGTCACG560700.038.2654928
TCGTATG555500.038.21483645
AGCACAC574650.037.86900310
ACACGTC581350.037.3203713
CTCGTAT572500.037.14295644
GTTTCGG597100.035.90651734
CACGTCT605000.035.87263514
GAGCACA616450.035.4690369
CACGTTT611650.035.2450431
TCGGAAG619950.035.1070483
CAGTCAC613900.035.086127
CGTCTGA627050.034.6004216
ACGTTTC630550.034.2417632
TTTCGGA627550.034.11408235
TCTCGTA629200.033.9781543
Produced by FastQC (version 0.11.2)