FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l05n01_nutrinetat_vegc53_mh-1_r2y_hn.3510000008a065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l05n01_nutrinetat_vegc53_mh-1_r2y_hn.3510000008a065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24244745
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC2850711.175805313687564TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC319350.041.76262745
ACGTCTG334100.041.23463415
GCACACG335050.041.17142511
CACACGT339650.040.58733712
CGTATGC330000.040.3292544
AGCACAC344950.040.01589610
TATCTCG334950.039.82055339
ACACGTC348400.039.60672813
CTCGTAT336950.039.57751542
CACGTCT350700.039.2700114
TCGTATG342400.038.9278543
GAGCACA360350.038.3744249
TCGGAAG362400.038.2346343
GATCGGA369600.037.4480061
TCTCGTA357450.037.35176541
CATTTTA360100.037.14559634
CAGTCAC365050.037.04495227
CGTCTGA372100.036.99944716
ATCGGAA377050.036.7662472
CTGAACT375200.036.66020219
Produced by FastQC (version 0.11.2)