FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l05n01_nutrinetat_vegc51_kn-0_r2y_hn.35100000089ffd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l05n01_nutrinetat_vegc51_kn-0_r2y_hn.35100000089ffd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13597641
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC1484771.0919320490958688TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC166900.041.3599245
ACGTCTG173750.040.73909415
GCACACG174800.040.64879211
CACACGT175750.040.37787612
CGTATGC171800.040.1279344
AGCACAC179450.039.6330810
CTCGTAT176150.039.16251842
TCGTATG178200.038.7624843
ACACGTC183450.038.70760713
CACGTCT184250.038.4662914
GAGCACA186100.038.2651989
TCGGAAG186850.037.9786343
TCTCGTA185100.037.3053741
GATCGGA190700.037.2412761
CGTCTGA192100.036.85927616
ATCGGAA195200.036.449452
CAGTCAC191900.036.32950627
ATGGCAT190050.036.16803435
GGCATCT191450.035.938837
AGAGCAC199650.035.6681868
Produced by FastQC (version 0.11.2)