FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l05n01_nutrinetat_vegc49_ge-0_r2y_hn.35100000089f69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l05n01_nutrinetat_vegc49_ge-0_r2y_hn.35100000089f69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20504756
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG2111971.0299903105406376TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC233300.041.6680233
ACGTCTG247950.041.10198215
ACGTGGC238600.040.86958332
GCCTTAT241700.040.61074437
GCACACG251500.040.477111
CACGTGG243600.040.2075431
TATCTCG246000.040.1751841
GTCACGT243950.039.94747529
CACACGT255550.039.84441412
CTCGTAT248450.039.61604744
AGTCACG249550.039.4569828
TCACGTG247900.039.37399730
TCGTATG249500.039.3691645
ACACGTC261250.039.00951813
GTGGCCT249950.038.92836834
AGCACAC262450.038.8568710
CACGTCT265500.038.39354714
GGCCTTA258750.037.97821436
GAGCACA271250.037.6957639
TCGGAAG276200.037.030473
Produced by FastQC (version 0.11.2)