FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l05n01_nutrinetat_vegc47_edi-0_r2y_hn.35100000089f26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l05n01_nutrinetat_vegc47_edi-0_r2y_hn.35100000089f26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23516128
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCC1961300.834023356225991TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG262840.11177010092818002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC226050.040.6011745
CAGGCGA226500.040.2482734
CCAGGCG227300.040.146233
ACGTCTG233600.040.1130115
CGTATGC231350.039.5795144
GCACACG238700.039.38788611
CACACGT243250.038.6881212
AGGCGAT236400.038.5532335
AGCACAC246250.038.2533310
GGCGATC238000.038.1617436
CTCGTAT240450.038.14707642
ACACGTC246650.038.03626613
ACCAGGC241100.038.0197232
TCGTATG243350.037.67399243
CACGTCT251300.037.29665414
GCGATCT248350.036.64380337
CGTCTGA263300.035.63101216
GAGCACA266050.035.5586179
TCTCGTA260700.035.27888541
CAGTCAC262700.035.247427