FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l05n01_nutrinetat_vegc45_ct-1_r2y_hn.35100000089ee1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l05n01_nutrinetat_vegc45_ct-1_r2y_hn.35100000089ee1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25196253
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCC448040.17782009094765003TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320590.12723717292408518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC67450.030.39903645
ACGTCTG71750.029.27934815
CGTATGC75750.026.9909244
GCACACG79950.026.72646711
CTCGTAT81600.025.16616642
CACACGT86050.025.11938312
ACACGTC86900.024.45955313
AGCACAC89350.023.96507810
TCGTATG86450.023.70226543
CACGTCT94050.022.52830714
TCTCGTA100600.020.5919841
TCGGAAG105500.020.5850893
GAGCACA107450.020.5142739
GATCGGA110050.019.5121571
CAGTCAC108250.019.28529727
CGTCTGA111900.018.99495116
TCCAGTC120450.017.91092725
AATCTCG115850.017.84250839
ATCGGAA121050.017.756862
CACTCAA118400.017.36324934
Produced by FastQC (version 0.11.2)