FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l05n01_nutrinetat_vegc43_col-0_r2y_hn.35100000089e6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l05n01_nutrinetat_vegc43_col-0_r2y_hn.35100000089e6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22862688
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1600400.7000051787436368TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC191150.040.61070345
ACGTCTG199700.039.63484215
CGTATGC199600.038.9222344
GCACACG203900.038.81842811
CACACGT208800.037.9074612
CTCGTAT205850.037.78418442
TATCTCG206700.037.72673839
TCGTATG208950.037.2128543
ACACGTC214450.036.95068413
AGCACAC215450.036.7687410
AGTCACT213100.036.75740428
CACGTCT216850.036.479514
TAGCTTA215950.036.10033834
ACTAGCT216700.035.9689532
CACTAGC218550.035.66525331
TCACTAG224650.034.8570430
GAGCACA229350.034.7168589
GCTTATC225750.034.57304436
TCGGAAG230500.034.373043
TCTCGTA227750.034.3089441
Produced by FastQC (version 0.11.2)