FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l05n01_nutrinetat_vegc41_bur-0_r2y_hn.35100000089daf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l05n01_nutrinetat_vegc41_bur-0_r2y_hn.35100000089daf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15596544
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT25800.013.0111821
CCCGGTT18900.011.9203331
GCGCCCG6050.011.8989326
CGGGCCT8150.011.8866921
GCGGGCA6400.011.6167351
GCCGGAT22400.011.1641361
CGCGTAT9800.011.03482151
CGGGGCG1450.006765022410.8762761
GGGCGCT3359.631549E-910.7603241
GGCGAAT24500.010.7589521
TCGGGGG13000.010.744082
GGCGCCC6500.010.38282725
GTCGGAT43750.010.3506641
GCGGGAT18000.010.1382431
ACGTCTG21500.010.04326815
CGGGGAT18850.010.0396391
CCGCGCG1800.00296533439.99677313
CGGGCAT19800.09.7855091
GCCCGTT10050.09.6394561
GCGGGGA6800.09.6081381
Produced by FastQC (version 0.11.2)