FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l05n01_nutrinetat_vegc39_bi-1_r2y_hn.35100000089d28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l05n01_nutrinetat_vegc39_bi-1_r2y_hn.35100000089d28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38013411
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA39500.020.38559535
CGTACGT43650.018.29285834
CCGTACG45800.017.72881933
GCGGGCA19800.017.2964441
GGGGGAT72550.015.8694521
ACGTCTG56100.015.395925515
GCGGGCC7550.015.2195451
GCGGGCT19750.015.0586221
TACGTAA53850.014.95322236
GCCGGAT52400.014.8342711
GCCGGTT74600.014.0138541
GCGGGTT83750.013.9355271
GCACACG64350.013.91143711
GTCGGAT122800.013.0451671
GGGCGCC4700.012.943281
CACACGT69200.012.77391312
GTCGGCT34450.012.6225331
GGGGATA92150.012.4689472
AGCACAC73550.012.44655710
ACCGTAC68500.012.11753832
Produced by FastQC (version 0.11.2)