FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l05n01_nutrinetat_vegc37_akita_r2y_hn.35100000089ca0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l05n01_nutrinetat_vegc37_akita_r2y_hn.35100000089ca0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27908675
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG1266940.45395920802402834TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG165600.036.79489533
ACGTCTG171050.036.30631615
GTCACGT168100.036.22552529
GCACACG174650.035.50643511
AGTCACG173850.035.0922728
TCGTATG176100.034.5314145
CACACGT180500.034.36813412
AGCACAC182450.034.0501210
ACACGTC187250.033.17727713
CTCGTAT184300.033.061544
CACGTCT194250.031.8543314
GTTTCGG191950.031.73214334
GAGCACA202300.030.8758499
CACGTTT199800.030.60155331
CAGTCAC201900.030.49604427
TCGGAAG207550.029.978853
CGTCTGA208400.029.81019816
TTTCGGA205050.029.77069335
ACGTTTC209100.029.46563532
GATCGGA213900.029.1250671
Produced by FastQC (version 0.11.2)