FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l04n01_nutrinetat_vegc36_tsu-0_r2o_ln.3510000008a220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l04n01_nutrinetat_vegc36_tsu-0_r2o_ln.3510000008a220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39107556
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC5744591.4689207374656703TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC631250.041.9259345
ACGTCTG661200.041.8180515
GCACACG663800.041.75590511
CGTATGC637950.041.4489344
ACACGTC677550.040.8520913
TCGTATG652200.040.5605543
TATCTCG653700.040.53998639
CACACGT684650.040.4678612
AGCACAC691750.040.1305410
CTCGTAT662650.039.89035842
CACGTCT695250.039.80235314
CGTCTGA691300.039.53201316
GAGCACA704200.039.465769
TCGGAAG711650.039.0963363
GATCGGA726000.038.252231
AGAGCAC726100.038.238268
CATTTTA697100.038.15478534
TCTCGTA699450.037.89772841
ATCGGAA737700.037.6760752
CAGTCAC714900.037.51535427
Produced by FastQC (version 0.11.2)