FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l04n01_nutrinetat_vegc34_stw-0_r2o_ln.3510000008a1b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l04n01_nutrinetat_vegc34_stw-0_r2o_ln.3510000008a1b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23107860
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC2819801.2202774294114644TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC321250.041.65490745
ACGTCTG332200.041.4885515
GCACACG334200.041.3613911
CGTATGC326750.040.8933844
ACACGTC342700.040.25675213
CACACGT343350.040.2132912
TCGTATG333650.040.09487543
AGCACAC345600.040.04910710
CACGTCT350700.039.32560714
CTCGTAT343450.038.9966542
TCGGAAG363400.038.2312973
GAGCACA363150.038.2251249
CGTCTGA361900.037.6673216
TCTCGTA356150.037.618741
CAGTCAC357750.037.4378927
GATCGGA372100.037.3656161
ATCGGAA375750.036.9447332
AGAGCAC378200.036.674278
CATGGCA364300.036.6721534
ATGGCAT365500.036.4717535
Produced by FastQC (version 0.11.2)