FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l04n01_nutrinetat_vegc32_st-0_r2o_ln.3510000008a13e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l04n01_nutrinetat_vegc32_st-0_r2o_ln.3510000008a13e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10473443
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG1392161.3292286022848456TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC155500.041.7999933
ACGTCTG163800.041.5626415
GCACACG163950.041.55204811
GTCACGT156200.041.22829
TCACGTG158650.040.84583330
ACACGTC167000.040.79316313
TCGTATG159750.040.78925745
CACGTGG159550.040.70004731
AGTCACG160400.040.65347728
GCCTTAT161850.040.47924437
CACACGT169000.040.31040612
ACGTGGC162100.040.1462232
TATCTCG163700.040.1316941
AGCACAC171400.039.81157710
GTGGCCT164500.039.51268434
CACGTCT172500.039.47947314
GGCCTTA165900.039.47749336
CTCGTAT166300.039.24729544
CGTCTGA171700.039.11326616
TCGGAAG177600.038.568113
Produced by FastQC (version 0.11.2)