FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l04n01_nutrinetat_vegc30_shahdara_r2o_ln.3510000008a0b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l04n01_nutrinetat_vegc30_shahdara_r2o_ln.3510000008a0b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16398079
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCC12137837.401982878604256TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1301850.044.09533745
ACGTCTG1355750.043.9953715
GCACACG1358950.043.99107411
CCAGGCG1301000.043.8812233
AGCACAC1363150.043.85058210
CAGGCGA1296650.043.83069634
ACACGTC1362750.043.8155913
CACACGT1364650.043.78590412
TCGGAAG1373300.043.6498263
CACGTCT1366750.043.64292514
GAGCACA1370950.043.622429
AGGCGAT1304150.043.57345635
CGTATGC1317600.043.553744
ACCAGGC1315550.043.5065532
CGTCTGA1353850.043.49559816
GATCGGA1383200.043.412511
ATCGGAA1381850.043.404212
TCGTATG1322350.043.3989543
GGCGATC1307350.043.39798436
AGAGCAC1380550.043.3353738
Produced by FastQC (version 0.11.2)