FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l04n01_nutrinetat_vegc28_sakata_r2o_ln.3510000008a008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l04n01_nutrinetat_vegc28_sakata_r2o_ln.3510000008a008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22135014
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCC5656232.5553315665397816TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC611850.043.0018945
ACGTCTG625000.042.90627315
GCACACG629400.042.73854411
ACACGTC631800.042.47295813
CGTATGC621600.042.27980444
CACACGT636800.042.1924512
AGCACAC640050.042.0871410
TCGTATG627400.041.9104643
CACGTCT646850.041.48475614
TCGGAAG651350.041.471363
CTCGTAT636650.041.29446842
GAGCACA654350.041.277379
GATCGGA655500.041.2444151
CGTCTGA643450.041.2390716
ATCGGAA661450.040.8347052
AGAGCAC664050.040.5965168
CAGTCAC651700.040.51872327
CGGAAGA668100.040.4856034
CACTCAA653350.040.35256234
TCTCGTA654800.040.18763741
Produced by FastQC (version 0.11.2)