FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l04n01_nutrinetat_vegc26_oy-0_r2o_ln.35100000089f5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l04n01_nutrinetat_vegc26_oy-0_r2o_ln.35100000089f5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544858
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC163921.0610683959302407TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC18150.042.20341545
ACGTCTG19150.041.45908415
GCACACG19200.041.2339811
CGTATGC18850.040.56779544
ACACGTC19600.040.3924713
TCGTATG19050.040.14188443
AGTCACT19150.039.83673528
TATCTCG19500.039.2155339
CTCGTAT19600.039.01545342
ACTAGCT19950.038.9009632
AGCACAC20600.038.65003210
TCACTAG19900.038.56027230
CACACGT20550.038.41573312
GTCACTA19950.038.12647229
TAGCTTA20100.037.93302534
CACTAGC20250.037.89379531
CACGTCT21150.037.538614
TCTCGTA20500.037.41229641
GAGCACA21500.037.3459559
GATCGGA21500.037.3174361
Produced by FastQC (version 0.11.2)