FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l04n01_nutrinetat_vegc24_n13_r2o_ln.35100000089ec7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l04n01_nutrinetat_vegc24_n13_r2o_ln.35100000089ec7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14685083
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC1904.3837645E-1016.6094361
GCACACG22700.012.88028111
GGCGCGC2704.5620254E-912.5229871
GCCGGTT24100.012.1592481
CGAGTGG22850.011.61458433
ACGTCTG24250.011.40778315
CGGGCAT19250.011.3585131
GGCGAAT27950.011.2102021
CGGGCCT9750.010.86611
CTCGGTT34850.010.801751
GCGGGCA8150.010.7866731
GCCGGAT28000.010.465641
GCGGGCC3257.050585E-810.4037131
GTCGGAT31250.010.2428031
ACGAGTG25850.010.1809432
TATCTCG25450.010.16290741
CCGGGAT20950.010.1140311
GGGGCCT6300.010.0183911
CCCGTGT14600.09.8811511
CCGGGTT34450.09.8802561
Produced by FastQC (version 0.11.2)