FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l04n01_nutrinetat_vegc22_mt-0_r2o_ln.35100000089e34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l04n01_nutrinetat_vegc22_mt-0_r2o_ln.35100000089e34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21312306
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT25100.023.38699234
GTACGTA25600.023.36948835
GGGCGCC2400.020.6638641
CCGTACG31700.019.36930333
ACGTCTG36100.018.56592815
GCACACG39300.017.45479811
GCGGGCT12350.016.7927151
TACGTAA36600.016.59167536
GCGCGCG8000.016.3415452
GCGGGCA10300.015.3200741
TCGTATG42150.014.81235345
ACCGTAC42800.014.45271732
ACACGTC45200.014.43002713
AGCACAC46900.014.19470610
CACACGT47450.014.17237112
GGGCGCT5300.014.0358311
ACGTAAT44500.013.94943537
GCGGGTT47200.013.7546821
GGGGGAT26100.013.7327231
GCCGGAT32700.013.6495231
Produced by FastQC (version 0.11.2)