FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l04n01_nutrinetat_vegc20_mr-0_r2o_ln.35100000089d92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l04n01_nutrinetat_vegc20_mr-0_r2o_ln.35100000089d92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31712133
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG1717640.5416349635011937TruSeq Adapter, Index 21 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG654130.20627120856235057No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA419130.13216707939513248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG216800.037.90674233
TCGTATG217600.037.6826945
CTCGTAT226500.036.2135144
AGTCACG228600.035.90766528
GTCACGT228700.035.78373329
ACGTCTG238850.035.0001915
TTCGGAA242500.033.8242236
CACGTTT245350.033.66591631
TTTCGGA244850.033.53632735
ACGTTTC246600.033.5041632
TCGGAAT253350.032.33127237
CAGTCAC255850.032.27853827
ACACGTC260400.032.181413
GTTTCGG256550.032.14716334
GCACACG261800.031.95776411
TCTCGTA259000.031.73013143
CACACGT267950.031.26623512
CACGTCT268900.031.13068214
AGCACAC274100.030.73702410
CGTCTGA276250.029.99303616
Produced by FastQC (version 0.11.2)