FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l03n01_nutrinetat_vegc8_col-0_r2o_ln.35100000089cf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l03n01_nutrinetat_vegc8_col-0_r2o_ln.35100000089cf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22208996
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC847770.38172369430837844TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC108150.036.8935345
CGTATGC110600.036.09705744
ACGTCTG115000.035.79133615
GCACACG117850.034.79218711
TCGTATG120450.033.257243
CACACGT124400.032.9964412
TATCTCG121550.032.9192239
ACACGTC125150.032.79870213
CTCGTAT126700.031.52788742
AGCACAC130650.031.52168810
TAGCTTA128700.031.4221434
AGTCACT131250.030.98157728
CACGTCT133950.030.81186514
ACTAGCT133200.030.3545632
CACTAGC136050.029.83882531
GAGCACA141450.029.4966349
CGTCTGA141350.029.15105616
TCGGAAG142150.029.0196553
GTCACTA141350.028.73591829
TCACTAG141650.028.53208430
Produced by FastQC (version 0.11.2)