FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l03n01_nutrinetat_vegc6_bur-0_r2o_ln.35100000089c93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l03n01_nutrinetat_vegc6_bur-0_r2o_ln.35100000089c93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10251678
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCA5550.013.3962211
ACGTCTG13550.012.94735515
GCACACG14600.012.32431711
GCGGGCG5500.011.85935414
GCCGGAT17400.011.6534541
CGGGCGC6050.011.52476515
ATCGGGC7300.011.40010456
GCGGGCC2604.76668E-711.2650051
GGCAGGT14950.011.0012751
CGGGCAT14150.010.9863651
GCGGGTT21200.010.9461841
GGGCGGC6350.010.6261379
GGCGGGC6350.010.62608413
CGCGTAT6150.010.6239061
CTCGGTT25350.010.3984661
GCGGGCT5200.010.3984661
CACACGT17200.010.33057212
GCCGGAC5950.010.22370151
ACGAGTG16750.010.07531532
TCGGGCG6900.09.7791267
Produced by FastQC (version 0.11.2)