FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l03n01_nutrinetat_vegc4_bi-1_r2o_ln.35100000089c79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l03n01_nutrinetat_vegc4_bi-1_r2o_ln.35100000089c79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17453363
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA21600.023.01237535
CCGTACG22850.021.7534933
CGTACGT23800.020.88517834
ACGTCTG27300.019.03146615
GCACACG31000.017.12273811
TACGTAA29400.016.83054736
ACCGTAC33000.015.4089932
GGGCGCT4000.015.2105471
TCGTATG33550.015.05011945
ACACGTC35550.014.48834413
GCGCGCG9200.014.4493372
CACACGT37350.014.33208112
GCCGGAT29250.014.3294041
ACGTAAT37350.013.72989337
AGCACAC39850.013.37666510
GCGGGCT8450.013.0671371
CTCGTAT40650.013.00263744
GCGGGCA10800.012.9362681
GGGCGCG1403.5650833E-412.8766541
GCCGGTT31550.012.8562471
Produced by FastQC (version 0.11.2)