FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l03n01_nutrinetat_vegc2_akita_r2o_ln.35100000089c50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l03n01_nutrinetat_vegc2_akita_r2o_ln.35100000089c50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24854382
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG2579381.0377968762208611TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG319250.040.28336715
GCACACG320550.040.26032611
CGTTTCG318800.039.82521433
AGTCACG322700.039.47052428
ACACGTC326800.039.33894313
GTCACGT324200.039.25990729
TCGTATG318800.039.23710645
CACACGT330500.038.95978512
AGCACAC331450.038.92978310
CTCGTAT328550.038.1575644
GTTTCGG336300.037.73277734
CACGTCT342200.037.62115514
GAGCACA345500.037.476879
CGTCTGA344900.037.4179416
CACGTTT348600.036.62798731
TCGGAAG355350.036.4204563
TTTCGGA348650.036.37038435
TTCGGAA347400.036.313536
CAGTCAC352100.036.283527
ACGTTTC353250.036.21051432
Produced by FastQC (version 0.11.2)