FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l03n01_nutrinetat_vegc18_mh-1_r2o_ln.35100000089f76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l03n01_nutrinetat_vegc18_mh-1_r2o_ln.35100000089f76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44871575
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC6666821.4857557373459702TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG771400.042.06768815
GTATGCC750350.041.98587845
GCACACG778250.041.82457711
CGTATGC763700.041.27270544
ACACGTC792800.040.96336413
TATCTCG778700.040.55565639
AGCACAC804200.040.50032810
CACACGT804050.040.47973312
TCGTATG778750.040.47796243
CACGTCT813300.039.94466814
CTCGTAT792250.039.81092542
TCGGAAG825650.039.469793
GAGCACA828550.039.3943149
GATCGGA831350.039.2007561
CGTCTGA835200.038.99960316
ATCGGAA852300.038.2907372
CATTTTA831450.038.277434
CAGTCAC838300.038.2359627
TCTCGTA827400.038.1468441
AGAGCAC862450.037.994518
Produced by FastQC (version 0.11.2)