FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l03n01_nutrinetat_vegc16_kn-0_r2o_ln.35100000089ed4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l03n01_nutrinetat_vegc16_kn-0_r2o_ln.35100000089ed4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17619179
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC2781091.578444716408182TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC309000.042.31541445
ACGTCTG321500.042.1002615
GCACACG323450.041.98552311
CGTATGC313450.041.68923644
ACACGTC328650.041.2390913
CACACGT332200.040.87964212
AGCACAC333500.040.84214810
TCGTATG320050.040.82953343
CACGTCT334050.040.5522514
CTCGTAT327500.039.90760442
GAGCACA344000.039.602059
CGTCTGA345050.039.3833216
TCGGAAG345250.039.351683
GATCGGA352250.038.603491
ATCGGAA352950.038.5080262
CATCTCG339900.038.3723239
TCTCGTA341650.038.2876841
CAGTCAC344950.038.28217327
AGAGCAC356500.038.276578
GGCATCT342000.038.13648237
Produced by FastQC (version 0.11.2)