FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l03n01_nutrinetat_vegc14_ge-0_r2o_ln.35100000089e5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l03n01_nutrinetat_vegc14_ge-0_r2o_ln.35100000089e5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25508563
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG2856271.1197298726706009TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG332300.041.36875515
CGTGGCC323700.041.182433
GCACACG339100.040.70511
ACGTGGC330650.040.5847732
CACGTGG334750.040.18011531
GCCTTAT333200.040.13648237
AGTCACG336750.040.12882628
ACACGTC345650.039.8165213
GTCACGT337600.039.7410129
TCACGTG338250.039.69782630
GGCCTTA339200.039.5525136
CACACGT348650.039.54487612
TATCTCG337600.039.52049641
TCGTATG340150.039.2223745
AGCACAC355250.038.9435310
GTGGCCT343950.038.8231934
CTCGTAT343950.038.8039544
CACGTCT357000.038.57584814
CGTCTGA359750.038.30597316
TCGGAAG363200.038.0685043
Produced by FastQC (version 0.11.2)