FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l03n01_nutrinetat_vegc12_edi-0_r2o_ln.35100000089de2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l03n01_nutrinetat_vegc12_edi-0_r2o_ln.35100000089de2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22285568
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCC3450571.5483428557890022TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGCG383700.042.5735133
GTATGCC384250.042.22195445
CAGGCGA385400.042.21064434
ACGTCTG395700.041.9417615
CGTATGC387250.041.88168744
GCACACG398700.041.69386711
GGCGATC393150.041.3442436
AGGCGAT394400.041.2531235
CACACGT403100.041.21086512
ACCAGGC399700.041.05920832
ACACGTC405150.040.98013713
TCGTATG398900.040.67543443
AGCACAC410700.040.5361510
GCGATCT402300.040.42066637
CTCGTAT401450.040.3890542
CACGTCT415000.040.02373514
CGTCTGA416450.039.9599916
TCGGAAG420300.039.5364843
GAGCACA426550.039.145899
GATCGGA424200.039.0627251
Produced by FastQC (version 0.11.2)