FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l03n01_nutrinetat_vegc10_ct-1_r2o_ln.35100000089d6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l03n01_nutrinetat_vegc10_ct-1_r2o_ln.35100000089d6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18056667
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCC3398451.882102605093177TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG196980.10908990014602363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC377550.042.64959745
ACGTCTG387950.042.5135615
GCACACG394600.042.0250911
CGTATGC383150.042.01922244
ACACGTC398550.041.49007413
CACACGT400900.041.30296712
TCGTATG390500.041.1707443
AGCACAC403850.041.08537310
CTCGTAT391900.040.97200842
CACGTCT405400.040.75573314
GAGCACA412700.040.2969789
TCGGAAG412300.040.2396133
CGTCTGA412700.040.19832616
GATCGGA415750.039.8402141
CAGTCAC410700.039.69755627
ATCGGAA420150.039.4703832
TCTCGTA409100.039.32087341
TCCAGTC420850.038.9589425
AGAGCAC428000.038.9563068
CCACTCA419400.038.91797633
Produced by FastQC (version 0.11.2)