FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l02n01_nutrinetat_vegc35_tsu-0_r2y_ln.35100000089f90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l02n01_nutrinetat_vegc35_tsu-0_r2y_ln.35100000089f90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17474773
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC3298751.8877212310569071TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC361900.042.6188145
ACGTCTG381050.042.49098615
GCACACG385000.042.1777211
CACACGT388600.041.8159212
AGCACAC390600.041.5960510
CGTATGC370700.041.5659844
TATCTCG371850.041.45557439
ACACGTC392700.041.3335213
TCGTATG376400.040.99030343
CTCGTAT377000.040.94892542
CACGTCT396350.040.91883514
TCGGAAG400550.040.4167443
GAGCACA404900.040.2769669
GATCGGA404550.039.9700051
CGTCTGA407450.039.74889816
TCTCGTA389950.039.6582541
ATCGGAA409500.039.541852
CAGTCAC399650.039.4659727
CATTTTA396150.039.0091934
CTGAACT415550.038.92120719
Produced by FastQC (version 0.11.2)