FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l02n01_nutrinetat_vegc33_stw-0_r2y_ln.35100000089f0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l02n01_nutrinetat_vegc33_stw-0_r2y_ln.35100000089f0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22278346
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC4262321.9132120490452926TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC476550.042.6700245
ACGTCTG495500.042.508415
GCACACG498850.042.3401611
CACACGT505750.041.6869112
CGTATGC489250.041.51141744
ACACGTC507900.041.50158713
AGCACAC509900.041.4270210
CTCGTAT493900.041.20711542
TCGTATG495100.041.06635343
CACGTCT515150.040.8345614
TCGGAAG519900.040.5867653
GAGCACA523400.040.418669
CGTCTGA527650.039.96096416
GATCGGA527500.039.91211
TCTCGTA511600.039.82981541
ATCGGAA532400.039.6059072
ATGGCAT512150.039.42254635
CAGTCAC521400.039.3289827
AGAGCAC539300.039.2687158
CTGAACT537050.039.15038719
Produced by FastQC (version 0.11.2)