FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l02n01_nutrinetat_vegc31_st-0_r2y_ln.35100000089e84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l02n01_nutrinetat_vegc31_st-0_r2y_ln.35100000089e84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41911544
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG5298211.26414097271148TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC586700.042.01059733
ACGTCTG610500.041.6062815
ACGTGGC597650.041.32660332
GTCACGT601750.041.03183429
GCCTTAT606700.040.8363337
CACGTGG607850.040.74658231
GCACACG627500.040.58303511
ACACGTC629800.040.3955513
AGTCACG619250.040.228628
TCACGTG615050.040.22456430
TATCTCG618100.040.1049941
TCGTATG617800.040.0694345
GGCCTTA619850.040.0425636
AGCACAC637000.040.00251410
CACACGT637150.039.92602512
GTGGCCT619750.039.83128434
CTCGTAT626650.039.46807544
CACGTCT645000.039.43661514
TCGGAAG651450.039.027523
GATCGGA661600.038.3343241
Produced by FastQC (version 0.11.2)