FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l02n01_nutrinetat_vegc29_shahdara_r2y_ln.35100000089e1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l02n01_nutrinetat_vegc29_shahdara_r2y_ln.35100000089e1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24067307
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCC8947693.7177778136955664TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268990.1117657243496333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC986800.043.71228445
GCACACG1045600.043.59020211
CCAGGCG984200.043.57028633
ACGTCTG1044650.043.563115
CAGGCGA977950.043.50082834
CACACGT1049150.043.4169812
AGCACAC1053350.043.2630810
ACACGTC1055550.043.15160413
CGTATGC1000900.043.0808844
ACCAGGC1003200.042.95120632
AGGCGAT991300.042.9059235
CACGTCT1061700.042.87410714
GGCGATC991050.042.83958436
GAGCACA1065950.042.800229
TCGTATG1009000.042.76847543
CTCGTAT1014250.042.57592442
TCGGAAG1066500.042.5691223
CGTCTGA1073200.042.3979216
CAGTCAC1038650.042.20000527
AGAGCAC1081200.042.1902968